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HOW TO OPERATE MICROBIOLOGICAL INCUBATORS

MICROBIOLOGICAL INCUBATORS

Laboratory incubators provide a controlled, contaminant-free environment for safe, reliable work with cell and tissue cultures by regulating conditions such as temperature, humidity, and CO2. Microbiological incubators are used for the growth and storage of bacterial cultures.

Types and sizes abound, including dry bath incubators with single or dual blocks, biological oxygen demand (BOD) units ideal for insect or plant studies, shaking incubators, hybridization ovens, bioreactors, and a wide variety of laboratory test chambers. Finding the correct size for your particular application is an easy task, with sizes ranging from small tabletop units to room-size. Laboratory incubators are essential for cell and tissue culture, biochemical and hematological studies, pharmaceutical work, and food analysis. Reducing contamination is one of the main goals in incubator design. To this end, manufacturers offer high-temperature decontamination cycles, HEPA filtration, and the use of antimicrobial copper components in the chamber. Some units feature inner glass doors that permit the contents to be viewed without disrupting the atmosphere of the incubator.

Integrated touch-screen displays, programmable alarms, data storage, and removable shelves are additional design improvements that focus on making laboratory incubators easy and more convenient to use.

OPERATING PROCEDURE
1. Ensure that the incubator is properly connected to the power supply.
2. Switch on the main.
3. Turn on the red color power knob towards 0-1.
4. Turn on the cooling knob towards 0-1.
5. To set the incubator at 22°C , set the lower temperature 21 OC by pressing the ‘SET POINT -1’ and simultaneously adjust the
temperature with the help of screw of SET and RST by screw driver.
6 .Set the higher temperature 23 OC by pressing the ‘SET POINT -2’ and simultaneously adjust the temperature with the help of screw of
SET and RST by screw driver.
7. In the same manner the incubator can be set to 37°, 44° and 55°C whenever required by setting the lower temperature to 36°,43° and 54° C respectively and by setting the higher temperature to 38°,45° and 56° C respectively.
8. Record the temperature twice daily. I.e. in the morning and in the evening. The temperature should not differ ± 2° C from the set
temperature.
9. Trouble shooting problems and remedial action:
10.1 the temperature display is not glowing. Check for power supply and proper electrical connections of instruments with power point.
11. Temperature is not even in the incubator. The air circulating fan may not be functioning. Check for it.
12. Report any discrepancy observed during operation or temperature monitoring to Q.C. Executive and notify the defect to Maintenance Department.

CALIBRATION

1. set the incubator temperature to 22°C. Wait till the set temperature is reached.
2. Take a calibrated thermometer and dip it in a 500 ml beaker filled to 3/4 of the volume with Glycerol AR grade.
3. Keep the beaker inside, at the center of the Microbiological Incubators. close the incubator door. Allow the temperature to equilibrate for 30 minutes.
4. Observe the temperature shown by the thermometer. The temperature shown by the
display and by thermometer shall not differ by more than 0.5°C.
5. Record the temperature at two time intervals ( with a gap of 6 hours) in the temperature record.
6. By following the same procedure as above carry out calibration by setting the incubator temperature to 37°C, 44°C and 55°C.
7. Record any discrepancy observed during operation or during temperature monitoring to Quality Control Executive and notify the defect to technical assistant for rectification. Affix “BREAK DOWN” label on the instrument.

FREQUENCY OF CALIBRATION:

1. once a month and after each maintenance job.
2. Record the calibration record in the format prescribed

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